Yahya, Mohd Fakharul Zaman Raja and Alias, Zazali and Karsani, Saiful Anuar (2017) Subtractive Protein Profiling of Salmonella typhimurium Biofilm Treated with DMSO. The Protein Journal, 36 (4). pp. 286-298. ISSN 1572-3887, DOI https://doi.org/10.1007/s10930-017-9719-9.
Full text not available from this repository.Abstract
Salmonellatyphimurium is an important biofilm-forming bacteria. It is known to be resistant to a wide range of antimicrobials. The present study was carried out to evaluate the effects of dimethyl sulfoxide (DMSO) against S. typhimurium biofilm and investigate whole-cell protein expression by biofilm cells following treatment with DMSO. Antibiofilm activities were assessed using pellicle assay, crystal violet assay, colony-forming unit counting and extracellular polymeric substance (EPS) matrix assay whilst differential protein expression was investigated using a combination of one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, tandem mass spectrometry and bioinformatics. Treatment with 32% DMSO inhibited pellicle formation, biofilm viability, biofilm biomass and several important components of EPS matrix. Subtractive protein profiling identified two unique protein bands (25.4 and 51.2 kDa) which were present only in control biofilm and not in 32% DMSO-treated biofilm. In turn, 29 and 46 proteins were successfully identified from the protein bands of 25.4 and 51.2 kDa respectively. Protein interaction network analysis identified several biological pathways to be affected, including glycolysis, PhoP–PhoQ phosphorelay signalling and flagellar biosynthesis. The present study suggests that DMSO may inhibit multiple biological pathways to control biofilm formation.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Biofilm; Dimethyl sulfoxide; Salmonella typhimurium; Subtractive protein profiling |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 21 Oct 2019 03:00 |
| Last Modified: | 21 Oct 2019 03:00 |
| URI: | http://eprints.um.edu.my/id/eprint/22766 |
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