Giribabu, Nelli and Karim, Kamarulzaman and Salleh, Naguib (2018) Effects of Marantodes pumilum (Kacip Fatimah) on vaginal pH and expression of vacoular ATPase and carbonic anhydrase in the vagina of sex-steroid deficient female rats. Phytomedicine, 49. pp. 95-105. ISSN 0944-7113, DOI https://doi.org/10.1016/j.phymed.2018.05.018.
Full text not available from this repository.Abstract
Background: In sex-steroid deficiency, increased in the pH of vaginal fluid is due to low estrogen levels. Hypothesis: Consumption of Marantodes pumilum leaves helps to ameliorate increased in vaginal fluid pH in sex-steroid deficient condition. Purpose: To investigate changes in vaginal fluid pH and expression of proteins that participate in pH changes i.e vacoular (V)-ATPases and carbonic anhydrases (CA) in the vagina following M. pumilum leaves consumption. Methods: Ovariectomized adult female rats were treated orally with M. pumilum leaves extract (MPE) at 100, 250 and 500 mg/kg.b.w and estradiol at 0.2 µg/kg/b.w for 28 days. At the end of the treatment, vaginal fluid pH was measured in anesthetised rats by using micropH probe. Following sacrificed, levels of V-ATPase and CA proteins and mRNAs in the vagina were identified by Western blotting and real-time PCR, respectively. Protein distribution was visualized by immunohistochemistry. Results: Administration of MPE causes the pH of vaginal fluid to decrease and expression and distribution of vaginal V-ATPase A & B and CA II, III, IX, XII and XIII to increase. Conclusions: The decrease in vaginal fluid pH following MPE treatment suggested that this herb has potential to be used to ameliorate vaginal fluid pH changes in sex-steroid deficient condition.
| Item Type: | Article |
|---|---|
| Funders: | NKEA grant, Ministry of Agriculture and Agro-based Industries, Malaysia, grant number ( GA014-2014 ) |
| Uncontrolled Keywords: | Marantodes pumilum; Vagina; pH, V-ATPase; Carbonic anhydrases |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 21 Aug 2019 07:38 |
| Last Modified: | 21 Aug 2019 07:38 |
| URI: | http://eprints.um.edu.my/id/eprint/22022 |
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