Halim, Nur Asniyati Abdul and Tan, Boon Chin and Midin, Mohd Razik and Madon, Maria and Khalid, Norzulaani and Yaacob, Jamilah Syafawati (2018) Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylase (HDAC) activity in Ananas comosus var. MD2. Plant Cell, Tissue and Organ Culture, 133 (1). pp. 123-135. ISSN 0167-6857, DOI https://doi.org/10.1007/s11240-017-1367-4.
Full text not available from this repository.Abstract
Somaclonal and phenotypic variation caused by genetic and/or epigenetic modifications, are a valuable source of genetic variation to improve desirable polygenetic traits in crops. In this study, we induced somaclonal variation in vitro pineapple (Ananas comosus var. MD2) through hormonal induction, NaCl, and abscisic acid (ABA) supplementation. Our results showed that supplementation of high concentration of 6-benzylaminopurine (4.0 mg/L BAP) alone or combined with indole-butyric acid (IBA) produced the highest percentage of dwarf variants (100%). Murashige and Skoog (MS) media containing 4.0 mg/L BAP plus 2.0 mg/L IBA produced the shortest plantlets (1.9 ± 0.1 cm). In comparison, MS media containing 1.0% NaCl induced formation of dwarf plantlets with a mean plantlet height of 1.4 ± 0.3 cm, whereas 1.0 mg/L ABA generated plantlets with a mean plantlet height of 1.7 ± 0.1 cm. We then analyzed the histone deacetylase (HDAC) enzyme activity for dwarf and non-dwarf plantlets. In general, dwarf plantlets exhibited higher HDAC activity than non-dwarf plantlets. The highest HDAC activity (109, 333.33 ± 4.40 ng/min/mg) was recorded for dwarf plantlets grown on media supplemented with 1.0 mg/L ABA. The dwarf variants also underwent phenotypic recovery to normal phenotype within 8 months after transferred to MS basal media. No ploidy alteration was detected in these dwarf plantlets after analyzed by flow cytometry. Taken together, although the generated dwarf plantlets showed higher HDAC activity compared to non-dwarf plantlets, their capability of reverting to non-dwarf phenotype suggested that it might be due to epigenetic modulation.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia: (FRGS Grant No. FP041-2014A), University of Malaya, Malaysia: PPP grant PG117-2015A |
| Uncontrolled Keywords: | Flow cytometry; Histone deacetylation; Phenotype recovery; Pineapple; Somaclonal variants |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 20 Aug 2019 04:15 |
| Last Modified: | 20 Aug 2019 04:15 |
| URI: | http://eprints.um.edu.my/id/eprint/21972 |
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