Phong, Win Nee and Show, Pau Loke and Le, Cheng Foh and Tao, Yang and Chang, Jo-Shu and Ling, Tau Chuan (2018) Improving cell disruption efficiency to facilitate protein release from microalgae using chemical and mechanical integrated method. Biochemical Engineering Journal, 135. pp. 83-90. ISSN 1369-703X, DOI https://doi.org/10.1016/j.bej.2018.04.002.
Full text not available from this repository.Abstract
One of the critical challenges in releasing protein from microalgae is to effectively disrupt their rigid thick cell walls. This study could provide some guidance on the implementation of a simple, cost-effective and scalable cell disruption in a downstream processing of microalgal industry for the recovery of protein from microalgae. The effects of solvent types, alkalis, and ultrasonication in disrupting microalgal cell wall and protein solubility were studied. It was found that alkaline treatment played a key role in cell disruption and protein solubilisation. From the industrial perspective, water is an excellent choice of extractive solvent due to low-cost, safety, and scalability. Among all the tested methods, the combination of both alkaline and ultrasonication treatment demonstrated the greatest cell disruption efficiency and was thus suggested to be use at large scale.
| Item Type: | Article |
|---|---|
| Funders: | SATU Joint Research Scheme ( RU018C-2016 , RU018L-2016 , RU022E-2014 ) from University of Malaya ( PG250-2015B ), Malaysia’s Fundamental Research Grant Scheme ( FRGS/1/2015/SG05/UNIM/03/1 ), Malaysia’s Ministry of Science, Technology, Innovation ( MOSTI-02-02-12-SF0256 ), Taiwan's Ministry of Science and Technology ( 104-3113-E-006-003 - and 103-2221-E-006-190-MY3 ), Taiwan's Ministry of Education on Top University Grants |
| Uncontrolled Keywords: | Microalgae; Protein; Alkaline treatment; Ultrasonication treatment; Cell disruption |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 19 Apr 2019 02:03 |
| Last Modified: | 16 Jun 2020 01:54 |
| URI: | http://eprints.um.edu.my/id/eprint/21025 |
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