Gan, Khai Swan and Tan, Chon Seng and Othman, Rofina Yasmin and Harikrishna, Jennifer Ann (2018) Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana. Sains Malaysiana, 47 (3). pp. 499-509. ISSN 0126-6039, DOI https://doi.org/10.17576/jsm-2018-4703-09.
Full text not available from this repository.Abstract
The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use.
| Item Type: | Article |
|---|---|
| Funders: | High Impact Research Chancellery Grant UM.C/625/1/ HIR/MOHE/SCI/14, CEBAR grant RU006-2017 from the University of Malaya, ABI fund under project code RB1086AB10 from the Ministry of Science, Technology and Innovation, Malaysia |
| Uncontrolled Keywords: | Biotechnology; KDEL; plants; tobacco PR1a; vaccine |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 04 Mar 2019 08:16 |
| Last Modified: | 04 Mar 2019 08:17 |
| URI: | http://eprints.um.edu.my/id/eprint/20589 |
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