Yew, Chee Hong and Azari, Pedram and Choi, Jane Ru and Li, Fei and Pingguan-Murphy, Belinda (2018) Electrospin-coating of nitrocellulose membrane enhances sensitivity in nucleic acid-based lateral flow assay. Analytica Chimica Acta, 1009. pp. 81-88. ISSN 0003-2670, DOI https://doi.org/10.1016/j.aca.2018.01.016.
Full text not available from this repository.Abstract
Point-of-care biosensors are important tools developed to aid medical diagnosis and testing, food safety and environmental monitoring. Paper-based biosensors, especially nucleic acid-based lateral flow assays (LFA), are affordable, simple to produce and easy to use in remote settings. However, the sensitivity of such assays to infectious diseases has always been a restrictive challenge. Here, we have successfully electrospun polycaprolactone (PCL) on nitrocellulose (NC) membrane to form a hydrophobic coating to reduce the flow rate and increase the interaction rate between the targets and gold nanoparticles-detecting probes conjugates, resulting in the binding of more complexes to the capture probes. With this approach, the sensitivity of the PCL electrospin-coated test strip has been increased by approximately ten-fold as compared to the unmodified test strip. As a proof of concept, this approach holds great potential for sensitive detection of targets at point-of-care testing.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya PPP grant (PG037-2015B), Ministry of Higher Education, Malaysia: FRGS (FP054-2015A), National Instrumentation Program (2013YQ190467), People's Republic of China |
| Uncontrolled Keywords: | Point-of-care; Paper-based biosensor; Nucleic acid testing; Electrospinning; Polycaprolactone; Nitrocellulose |
| Subjects: | R Medicine |
| Divisions: | Faculty of Engineering |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Feb 2019 08:33 |
| Last Modified: | 12 Feb 2019 08:33 |
| URI: | http://eprints.um.edu.my/id/eprint/20274 |
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