Zakaria, Nurhisyam and Mahdzir, Mohamad and Yusoff, Mahfuzah and Arshad, Norhafiza Mohd and Awang, Khalijah and Nagoor, Noor Hasima (2018) Cytotoxic Effects of Pinnatane A Extracted from Walsura pinnata (Meliaceae) on Human Liver Cancer Cells. Molecules, 23 (11). p. 2733. ISSN 1420-3049, DOI https://doi.org/10.3390/molecules23112733.
Full text not available from this repository.Abstract
Background: Pinnatane A from the bark of Walsura pinnata was investigated for its anti-cancer properties by analyzing the cytotoxic activities and cell cycle arrest mechanism induced in two different liver cancer cell lines. Methods: A 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to analyze the pinnatane A selectivity in inducing cell death in cancer and normal cells. Various biological assays were carried out to analyze the anti-cancer properties of pinnatane A, such as a live/dead assay for cell death microscopic visualization, cell cycle analysis using propidium iodide (PI) to identify the cell cycle arrest phase, annexin V-fluorescein isothiocyanate (annexin V-FITC)/PI flow cytometry assay to measure percentage of cell populations at different stages of apoptosis and necrosis, and DNA fragmentation assay to verify the late stage of apoptosis. Results: The MTT assay identified pinnatane A prominent dose- and time-dependent cytotoxicity effects in Hep3B and HepG2 cells, with minimal effect on normal cells. The live/dead assay showed significant cell death, while cell cycle analysis showed arrest at the G0/G1 phase in both cell lines. Annexin V-FITC/PI flow cytometry and DNA fragmentation assays identified apoptotic cell death in Hep3B and necrotic cell death in HepG2 cell lines. Conclusions: Pinnatane A has the potential for further development as a chemotherapeutic agent prominently against human liver cells.
| Item Type: | Article |
|---|---|
| Funders: | Centre for Research in Biotechnology for Agriculture Research Grant [RU015-2015 and RU006-2017], Postgraduate Research Grant [PG044-2016A] |
| Uncontrolled Keywords: | anti-cancer; apoptosis; cell cycle arrest; necrosis; triterpene |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history |
| Divisions: | Faculty of Science Faculty of Science > Department of Chemistry Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 28 Jan 2019 03:23 |
| Last Modified: | 28 Jan 2019 03:23 |
| URI: | http://eprints.um.edu.my/id/eprint/20186 |
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