Hessami, Mohammad Javad and Cheng, Sit Foon and Ambati, Ranga Rao and Yeong, Hui Yin and Phang, Siew Moi (2019) Bioethanol production from agarophyte red seaweed, Gelidium elegans, using a novel sample preparation method for analysing bioethanol content by gas chromatography. 3 Biotech, 9 (1). p. 25. ISSN 2190-572X, DOI https://doi.org/10.1007/s13205-018-1549-8.
Full text not available from this repository.Abstract
In this study, Gelidium elegans is investigated for ethanol production. A combination of factors including different temperatures, acid concentration and incubation time was evaluated to determine the suitable saccharification conditions. The combination of 2.5% (w/v) H2SO4 at 120 °C for 40 min was selected for hydrolysis of the seaweed biomass, followed by purification, and fermentation to yield ethanol. The galactose and glucose were dominant reducing sugars in the G. elegans hydrolysate and under optimum condition of dilute acid hydrolysis, 39.42% of reducing sugars was produced and fermentation resulted in ethanol concentration of 13.27 ± 0.47 g/L. A modified method was evaluated for sample preparation for gas chromatography (GC) analysis of the ethanol content. A solvent mixture of acetonitrile and iso-butanol precipitated dissolved organic residues and reduced water content in GC samples at least by 90%. Results showed that this method could be successfully used for bioethanol production from seaweed.
| Item Type: | Article |
|---|---|
| Funders: | University of Malaya Research Grant (Project no: PV026-2012), GC002B-15SBS and HICoE Grant IOES-2014F |
| Uncontrolled Keywords: | Bioethanol; G. elegans; Gas chromatography; Sample preparation; Seaweed |
| Subjects: | Q Science > Q Science (General) Q Science > QD Chemistry Q Science > QH Natural history |
| Divisions: | Faculty of Science > Department of Chemistry Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 22 Jan 2019 01:47 |
| Last Modified: | 22 Jan 2019 01:47 |
| URI: | http://eprints.um.edu.my/id/eprint/20073 |
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