Tan, Suh Nih and Lim, Hong Chang and Teng, Sing Tung and Lim, Po Teen and Leaw, Chui Pin (2015) Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA. Fisheries Science, 81 (5). pp. 831-838. ISSN 0919-9268, DOI https://doi.org/10.1007/s12562-015-0902-7.
Full text not available from this repository.Abstract
An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrial-encoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1–8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.
| Item Type: | Article |
|---|---|
| Funders: | Malaysian Government through the Ministry of Education under KPM-JSPS COMSEA Matching Fund (GA002-2014), Higher Institution Centre of Excellence Research Grant (IOES-2014C), University of Malaya Research Program (RU006A/B-2014) |
| Uncontrolled Keywords: | cox1; Diatom; LSU rDNA; Primer; Pseudo-nitzschia |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 05 Oct 2018 05:28 |
| Last Modified: | 05 Oct 2018 05:28 |
| URI: | http://eprints.um.edu.my/id/eprint/19614 |
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