Thirugnanasampandan, R. and Ramya, G. and Gogulramnath, M. and Rajarajeswaran, J. and Kanthimathi, M.S. (2014) Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil. Pharmacognosy Journal, 7 (1). pp. 32-36. ISSN 0975-3575, DOI https://doi.org/10.5530/pj.2015.7.3.
Full text not available from this repository.Abstract
Introduction: Plectranthus amboinicus(Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53±0.01 and 87±0.01μg/mL respectively. At 200μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Essential oil; Cytotoxicity; Antigenotoxicity; Zymogram; RT- PCR |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Oct 2018 03:25 |
| Last Modified: | 03 Oct 2018 03:25 |
| URI: | http://eprints.um.edu.my/id/eprint/19550 |
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