Wong, W.Y. and Loh, S.W. and Ng, W.L. and Tan, M.C. and Yeo, K.S. and Looi, C.Y. and Maah, M.J. and Ea, C.K. (2015) A cell-based screening system for anti-influenza A virus agents. Scientific Reports, 5 (1). p. 8672. ISSN 2045-2322, DOI https://doi.org/10.1038/srep08672.
Full text not available from this repository.Abstract
Emerging of drug resistant influenza A virus (IAV) has been a big challenge for anti-IAV therapy. In this study, we describe a relatively easy and safe cell-based screening system for anti-IAV replication inhibitors using a non-replicative strain of IAV. A nickel (II) complex of polyhydroxybenzaldehyde N4-thiosemicarbazone (NiPT5) was recently found to exhibit anti-inflammatory activity in vivo and in vitro. NiPT5 impedes the signaling cascades that lead to the activation of NF-κB in response to different stimuli, such as LPS and TNFα. Using our cell-based screening system, we report that pretreating cells with NiPT5 protects cells from influenza A virus (IAV) and vesicular stomatitis virus (VSV) infection. Furthermore, NiPT5 inhibits replication of IAV by inhibiting transcription and translation of vRNAs of IAV. Additionally, NiPT5 reduces IAV-induced type I interferon response and cytokines production. Moreover, NiPT5 prevents activation of NF-κB, and IRF3 in response to IAV infection. These results demonstrate that NiPT5 is a potent antiviral agent that inhibits the early phase of IAV replication.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Antiviral Agents; Cell Line, Tumor; Cytokines; Drug Evaluation, Preclinical; Humans; Influenza A virus; Interferon Regulatory Factor-3; Interferons; NF-kappa B; Virus Replication |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history R Medicine |
| Divisions: | Faculty of Medicine Faculty of Science > Department of Chemistry Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 26 Sep 2018 07:57 |
| Last Modified: | 26 Sep 2018 07:57 |
| URI: | http://eprints.um.edu.my/id/eprint/19434 |
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