Wong, Y.H. and Kadir, H.A. and Tayyab, S. (2015) Targeting chemical and thermal stability of ovalbumin by simulated honey sugar cocktail. International Journal of Biological Macromolecules, 73. pp. 207-214. ISSN 0141-8130, DOI https://doi.org/10.1016/j.ijbiomac.2014.11.015.
Full text not available from this repository.Abstract
Effect of simulated honey sugar cocktail (SHSC) on chemical and thermal stability of ovalbumin (OVA) was investigated using multiple-spectroscopic techniques. Urea-induced denaturation of OVA produced a transition, characterized by the start-, the mid- and the end-points at 3.2M, 5.9/5.6M and 8.5/8.0M urea, respectively, when studied by MRE222nm and tryptophan fluorescence measurements. Presence of 10% or 20% (w/v) SHSC in the incubation mixture shifted the transition curve towards higher urea concentration in a concentration dependent manner. A comparison of far- and near-UV CD, UV-difference, ANS fluorescence and 3-D fluorescence spectral results of native OVA and 5.9M urea-denatured OVA (U-OVA), obtained in the absence and the presence of 20% (w/v) SHSC suggested SHSC-induced stabilization of U-OVA. Furthermore, a significant shift towards higher denaturant concentration was also noticed in the GdnHCl and thermal transition curves of OVA in the presence of 20% (w/v) SHSC. Taken together, all these results suggested stabilization of OVA against chemical and thermal denaturations by SHSC.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Higher Education, Malaysia: HIR grant (UM.C/625/1/HIR/MoE/SC/02), Postgraduate Research Grant (PG072-2013B) |
| Uncontrolled Keywords: | Chemical denaturation; Thermal denaturation; Protein stabilization; Ovalbumin; Simulated honey sugar cocktail |
| Subjects: | Q Science > Q Science (General) Q Science > QH Natural history |
| Divisions: | Faculty of Science > Institute of Biological Sciences |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 26 Sep 2018 07:45 |
| Last Modified: | 26 Sep 2018 07:45 |
| URI: | http://eprints.um.edu.my/id/eprint/19432 |
Actions (login required)
 |
View Item |
