Khosravi, Y. and Tay, S.T. and Vadivelu, J. (2011) Analysis of integrons and associated gene cassettes of metallo-{beta}-lactamase-positive Pseudomonas aeruginosa in Malaysia. Journal of Medical Microbiology, 60 (pt7). pp. 988-1092. ISSN 0022-2615, DOI 21436370.
Full text not available from this repository.Abstract
In this study, 90 non-replicate imipenem-resistant Pseudomonas aeruginosa (IRPA) Malaysian isolates collected between October 2005 and March 2008 were subjected to a screening test for detection of the integron and the gene cassette. Class 1 integrons were detected in 54 IRPA clinical isolates, whilst three isolates contained class 2 integrons. Analysis of the gene cassettes associated with the class 1 integrons showed the detection of accC1 in isolates carrying bla(IMP-7) and aacA7 in isolates carrying bla(VIM-2). aadA6 was detected in two isolates carrying bla(IMP-4). Using random amplification of polymorphic DNA analysis, 14 PCR fingerprint patterns were generated from the 32 isolates carrying metallo-β-lactamase (MBL) genes (35.5 %), whilst 20 patterns were generated from the 58 non-MBL gene isolates (64.4 %). Based on the differences in the fingerprinting patterns, two clusters (A and B) were identified among the MBL-producing isolates. Cluster A comprised 18 isolates (56 %) carrying the bla(VIM) gene, whereas cluster B comprised 14 (44 %) isolates carrying the bla(IMP) gene. The non-MBL isolates were divided into clusters C and D. Cluster C comprised 22 non-MBL isolates harbouring class 1 integrons, whilst cluster D consisted of three isolates carrying class 2 integrons. These findings suggest that the class 1 integron is widespread among P. aeruginosa isolated in Malaysia and that characterization of cassette arrays of integrons will be a useful epidemiological tool to study the evolution of multidrug resistance and the dissemination of antibiotic resistance genes.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia |
| Uncontrolled Keywords: | Imipenem-resistant Pseudomonas Aeruginosa (IRPA) |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Mr. Faizal Hamzah |
| Date Deposited: | 05 Aug 2011 00:28 |
| Last Modified: | 26 Dec 2014 05:12 |
| URI: | http://eprints.um.edu.my/id/eprint/1937 |
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