Castaño-Rodríguez, N. and Goh, K.L. and Fock, K.M. and Mitchell, H.M. and Kaakoush, N.O. (2017) Dysbiosis of the microbiome in gastric carcinogenesis. Scientific Reports, 7 (1). p. 15957. ISSN 2045-2322, DOI https://doi.org/10.1038/s41598-017-16289-2.
Full text not available from this repository.Abstract
The gastric microbiome has been proposed as an etiological factor in gastric carcinogenesis. We compared the gastric microbiota in subjects presenting with gastric cancer (GC, n = 12) and controls (functional dyspepsia (FD), n = 20) from a high GC risk population in Singapore and Malaysia. cDNA from 16S rRNA transcripts were amplified (515F-806R) and sequenced using Illumina MiSeq 2 × 250 bp chemistry. Increased richness and phylogenetic diversity but not Shannon's diversity was found in GC as compared to controls. nMDS clustered GC and FD subjects separately, with PERMANOVA confirming a significant difference between the groups. H. pylori serological status had a significant impact on gastric microbiome α-diversity and composition. Several bacterial taxa were enriched in GC, including Lactococcus, Veilonella, and Fusobacteriaceae (Fusobacterium and Leptotrichia). Prediction of bacterial metabolic contribution indicated that serological status had a significant impact on metabolic function, while carbohydrate digestion and pathways were enriched in GC. Our findings highlight three mechanisms of interest in GC, including enrichment of pro-inflammatory oral bacterial species, increased abundance of lactic acid producing bacteria, and enrichment of short chain fatty acid production pathways.
| Item Type: | Article |
|---|---|
| Funders: | National Health and Medical Research Council, Australia Early Career fellowship (APP1111461), Cancer Australia priority-driven collaborative cancer research grant (1129488), Cancer Institute NSW Career Development Fellowship (15/CDF/1-11), Ministry of Higher Education, Malaysia (UM.C/625/HIR/MOHE/CHAN/13/1) , University of New South Wales |
| Uncontrolled Keywords: | Dysbiosis; Microbiome; Gastric carcinogenesis |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 13 Sep 2018 03:47 |
| Last Modified: | 13 Sep 2018 03:47 |
| URI: | http://eprints.um.edu.my/id/eprint/19217 |
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