Drewes, J.L. and White, J.R. and Dejea, C.M. and Fathi, P. and Iyadorai, T. and Vadivelu, J. and Roslani, A.C. and Wick, E.C. and Mongodin, E.F. and Loke, M.F. and Thulasi, K. and Gan, H.M. and Goh, K.L. and Chong, H.Y. and Kumar, S. and Wanyiri, J.W. and Sears, C.L. (2017) High-resolution bacterial 16S rRNA gene profile meta-analysis and biofilm status reveal common colorectal cancer consortia. npj Biofilms and Microbiomes, 3 (1). p. 34. ISSN 2055-5008, DOI https://doi.org/10.1038/s41522-017-0040-3.
Full text not available from this repository.Abstract
Colorectal cancer (CRC) remains the third most common cancer worldwide, with a growing incidence among young adults. Multiple studies have presented associations between the gut microbiome and CRC, suggesting a link with cancer risk. Although CRC microbiome studies continue to profile larger patient cohorts with increasingly economical and rapid DNA sequencing platforms, few common associations with CRC have been identified, in part due to limitations in taxonomic resolution and differences in analysis methodologies. Complementing these taxonomic studies is the newly recognized phenomenon that bacterial organization into biofilm structures in the mucus layer of the gut is a consistent feature of right-sided (proximal), but not left-sided (distal) colorectal cancer. In the present study, we performed 16S rRNA gene amplicon sequencing and biofilm quantification in a new cohort of patients from Malaysia, followed by a meta-analysis of eleven additional publicly available data sets on stool and tissue-based CRC microbiota using Resphera Insight, a high-resolution analytical tool for species-level characterization. Results from the Malaysian cohort and the expanded meta-analysis confirm that CRC tissues are enriched for invasive biofilms (particularly on right-sided tumors), a symbiont with capacity for tumorigenesis (Bacteroides fragilis), and oral pathogens including Fusobacterium nucleatum, Parvimonas micra, and Peptostreptococcus stomatis. Considered in aggregate, species from the Human Oral Microbiome Database are highly enriched in CRC. Although no detected microbial feature was universally present, their substantial overlap and combined prevalence supports a role for the gut microbiota in a significant percentage (>80%) of CRC cases.
| Item Type: | Article |
|---|---|
| Funders: | National Institutes of Health: Grants R01 CA151393 (to CLS), P30 DK089502 (GI Core), P30 CA006973 (Sidney Kimmel Comprehensive Cancer Center core), P50 CA062924 (SPORE in Gastrointestinal Cancers), University of Malaya Research Grant RP016A-13HTM, NIH Shared Instrumentation Grant S10OD016374 for the Zeiss 780 |
| Uncontrolled Keywords: | High-resolution; Bacterial; 16S rRNA gene; Profile meta-analysis; Biofilm; Status; Common; Colorectal cancer consortia |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 12 Sep 2018 02:34 |
| Last Modified: | 12 Sep 2018 02:34 |
| URI: | http://eprints.um.edu.my/id/eprint/19189 |
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