Phyu, W.K. and Ong, Kien Chai and Kong, C.K. and Khalil, A.A. and Ramanujam, T.M. and Wong, Kum Thong (2017) Squamous epitheliotropism of Enterovirus A71 in human epidermis and oral mucosa. Scientific Reports, 7 (1). p. 45069. ISSN 2045-2322, DOI https://doi.org/10.1038/srep45069.
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Abstract
Hand-foot-and-mouth disease is a self-limiting paediatric infectious disease commonly caused by Enterovirus A71 (Genus: Enterovirus, Family: Picornaviridae). Typical lesions in and around the hands, feet, oral cavity and other places may rarely be complicated by acute flaccid paralysis and acute encephalomyelitis. Although virus is readily cultured from skin vesicles and oral secretions, the cellular target/s of Enterovirus A71 in human skin and oral mucosa are unknown. In Enterovirus A71-infected human skin and oral mucosa organotypic cultures derived from the prepuce and lip biopsies, focal viral antigens and viral RNA were localized to cytoplasm of epidermal and mucosal squamous cells as early as 2 days post-infection. Viral antigens/RNA were associated with cytoplasmic vacuolation and cellular necrosis. Infected primary prepuce epidermal keratinocyte cultures showed cytopathic effects with concomitant detection of viral antigens from 2 days post-infection. Supernatant and/or tissue homogenates from prepuce skin organotypic cultures and primary prepuce keratinocyte cultures showed viral titres consistent with active viral replication. Our data strongly support Enterovirus A71 squamous epitheliotropism in the human epidermis and oral mucosa, and suggest that these organs are important primary and/or secondary viral replication sites that contribute significantly to oral and cutaneous viral shedding resulting in person-to-person transmission, and viraemia, which could lead to neuroinvasion.
| Item Type: | Article |
|---|---|
| Funders: | High Impact Research (HIR) UM.C/625/1/HIR-MOHE, Ministry of Higher Education, Malaysia: FP038/2015A FRGS |
| Uncontrolled Keywords: | Squamous epitheliotropism; Enterovirus A71; Human epidermis; Oral mucosa |
| Subjects: | R Medicine |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 03 Sep 2018 06:46 |
| Last Modified: | 13 Mar 2019 04:43 |
| URI: | http://eprints.um.edu.my/id/eprint/19061 |
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