Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia

Thong, Kwai Lin and Tan, Lai Kuan and Ooi, Peck Toung (2017) Genetic diversity, virulotyping and antimicrobial resistance susceptibility of Yersinia enterocolitica isolated from pigs and porcine products in Malaysia. Journal of the Science of Food and Agriculture, 98 (1). pp. 87-95. ISSN 0022-5142, DOI https://doi.org/10.1002/jsfa.8442.

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Abstract

BACKGROUND: The objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of Yersinia enterocolitica isolated from uncooked porcine food and live pigs in Malaysia. RESULTS: Thirty-two non-repeat Y. enterocolitica strains of three bioserotypes (3 variant/O:3, n = 27; 1B/O:8, n = 3; 1A/O:5, n = 2) were analysed. Approximately 90% of strains were multidrug-resistant with a multiple antibiotic resistance index < 0.2 and the majority of the strains were resistant to nalidixic acid, clindamycin, ampicillin, ticarcillin, tetracycline and amoxicillin. Yersinia enterocolitica could be distinguished distinctly into three clusters by pulsed-field gel electrophoresis, with each belonging to a particular bioserotype. Strains of 3 variant/O:3 were more heterogeneous than others. Eleven of the 15 virulence genes tested (hreP, virF, rfbC, myfA, sat, inv, ail, ymoA, ystA, tccC, yadA) and pYV virulence plasmid were present in all the bioserotpe 3 variant/03 strains. CONCLUSION: The occurrence of virulent strains of Y. enterocolitica in pigs and porcine products reiterated that pigs are important reservoirs for Y. enterocolitica. The increasing trend of multidrug resistant strains is a public health concern. This is the first report on the occurrence of potential pathogenic and resistant strains of Y. enterocolitica in pigs in Malaysia.

Item Type:	Article
Funders:	Univeristy of Malaya
Uncontrolled Keywords:	Antimicrobial profiles; Food microbiology; PFGE; Pig; Virulence; Yersinia enterocolitica
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Prof. Dr Kwai Lin Thong
Date Deposited:	13 Nov 2017 02:31
Last Modified:	21 Mar 2019 06:32
URI:	http://eprints.um.edu.my/id/eprint/18224

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