Zinc Content in Cord Blood Is Associated with Maternal Age and Parity

Youssof, A.L. and Abu Kassim, N.L. and Rashid, S.A. and De Ley, M. and Rahman, M.T. (2017) Zinc Content in Cord Blood Is Associated with Maternal Age and Parity. Biological Trace Element Research, 175 (1). pp. 17-26. ISSN 0163-4984, DOI https://doi.org/10.1007/s12011-016-0760-x.

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Abstract

At childbirth (parturition), zinc (Zn) homeostasis in cord blood (CB) can be affected by a number of factors: Zn in maternal blood, parturition related stress as well as metallothionein (MT). Both Zn and stress are known inducers of MT which is primarily involved in Zn homeostasis. This study analyzed Zn concentration [Zn], in CB components and MT-2A transcription in CB mononuclear cells (MNC) in relation to primiparous and multiparous childbirth. [Zn] in CB (n = 47) plasma, erythrocytes, and MNCs were measured by atomic absorption spectrophotometry (lambda = 213.9 nm). The MT-2A transcription in CB-MNC was quantified using real-time PCR. Significant correlations (Pearson r) were found between: plasma-[Zn] and erythrocyte-[Zn] (p = 0.002); [Zn] and MT-2A messenger RNA (mRNA) (p = 0.000) in CB-MNC. Student's t tests showed higher levels of MT-2A mRNA and MNC-[Zn] in CB of older (25 years) compared to younger mothers (24 years) (p = 0.043 and p = 0.016, respectively). Significantly higher [Zn] was found in CB plasma (p = 0.017) and MNC (p = 0.041) of older primiparous compared to the younger primiparous and older multiparous mothers respectively. MT-2A mRNA in CB-MNC was significantly lower in CB of younger primiparous mothers compared to their older counterparts (p = 0.001). Path analysis showed that MNC-[Zn] (beta = 0.83; p = 0.000) had a greater influence on MT-2A mRNA expression, compared to parity (beta = -0.14; p = 0.033). Higher [Zn] in CB of primiparous mothers could be linked to higher stress during parturition, however, might be beneficial for the growth and development of the child. Together MNC-[Zn] and parity contributed similar to 70 % of the MT-2A transcription in CB-MNC.

Item Type:	Article
Funders:	The grant of Basic and Applied Biomedical Research Cluster unit (RCG-22-05)
Uncontrolled Keywords:	Catecholamines; Erythrocytes; Mononuclear cells; Parturition; Plasma; Stress; Gene-expression; Estrogen-receptor; Leukocyte subsets; 1st childbirth; Trace-element; Plasma zinc; Pregnancy; Metallothionein; Women; Homeostasis; Biochemistry & Molecular Biology; Endocrinology & Metabolism
Subjects:	R Medicine > RK Dentistry > Practice of dentistry. Dental economics
Divisions:	Faculty of Dentistry
Depositing User:	Mr Ahmad Azwan Azman
Date Deposited:	26 Oct 2017 01:01
Last Modified:	26 Oct 2017 01:01
URI:	http://eprints.um.edu.my/id/eprint/18109

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