Lin, Y.K. and Show, P.L. and Yap, Y.J. and Tan, C.P. and Ng, E.P. and Ariff, A.B. and Annuar, M.S.M. and Ling, T.C. (2015) Direct recovery of cyclodextringlycosyltransferase from Bacillus cereus using aqueous two-phase flotation. Journal of Bioscience and Bioengineering , 120 (6). pp. 684-689. ISSN 1389-1723,
Full text not available from this repository.Abstract
Purification of cyclodextrin glycosyl transferase (CGTase) from Bacillus cereus using polyethylene glycol (PEG)-potassium phosphates aqueous two-phase flotation (ATPF) system was studied in this paper. The effects of varying PEG molecular weight, tie-line length (TLL) value, volume ratio (V-R), pH value, crude concentration and gas nitrogen flotation time were investigated. The optimal condition for purification of CGTase was attained at 18.0% (w/w) PEG 8000, 7.0% (w/w) potassium phosphates, V-R of 3.0, 20% (w/w) crude load at pH 7, and 80 min nitrogen flotation time at a flow rate of 5 L/min. With this optimal condition, purification factor (P-FT) of 21.8 and a yield (Y-T) of 97.1% were attained. CGTase was successfully purified in a single downstream processing step using the ATPF. (c) 2015, The Society for Biotechnology, Japan. All rights reserved.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Cyclodextringlycosyltransferase; Aqueous two-phase flotation; Bacillus cereus; Purification; Polyethylene glycol-salt system |
| Subjects: | Q Science > QR Microbiology |
| Depositing User: | Mrs. Siti Mawarni Salim |
| Date Deposited: | 15 Sep 2016 08:06 |
| Last Modified: | 15 Sep 2016 08:06 |
| URI: | http://eprints.um.edu.my/id/eprint/16468 |
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