Puthucheary, S.D. and Anuar, A.S.S. and Tee, T.S. (2010) Burkholderia thailandensis whole cell antigen cross-reacts with B. pseudomallei antibodies from patients with melioidosis in an immunofluorescent assay. Southeast Asian Journal of Tropical Medicine and Public Health, 41 (2). pp. 395-400.
Full text not available from this repository.Abstract
An immunofluorescent assay (I FAT) using whole cell antigen derived from Burkholderia thailandensis used for detection of total antibodies to Burkholderia pseudomallei, was found to compare favorably with a previous published report on a 13 pseudomallei IFAT assay. At a 1.20 cut-off titer, the assay had high sensitivity (98 9%) and satisfactory specificity (92.3%), when tested against sera from 94 patients suspected of melioidosis. Sera from 12 patients with culture proven melioidosis gave absolute concordance with the 2 test antigens No sera from 50 blood donors had a titer >= 20 of Cross-reactivity with patients' sera positive for Chlamydia, Mycoplasma, Legionella and typhoid was not observed, except for 3 sera from typhus patients and one from a patient with leptospirosis The major advantage of this assay is that the cultivation and preparation of B. thailandensis as antigen can be carried out in any laboratory with basic microbiological set-up. The serodiagnosis of melioidosis can be made safe for medical laboratory personnel, particularly in B pseudomallei endemic regions.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Burkholderia thailandensis; B pseudomallei; whole cell antigen; melioidosis; IFAT |
| Subjects: | R Medicine |
| Depositing User: | Dr Mohd Faizal Hamzah |
| Date Deposited: | 22 Dec 2015 02:15 |
| Last Modified: | 22 Dec 2015 02:15 |
| URI: | http://eprints.um.edu.my/id/eprint/15184 |
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