Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources

Teh, Cindy Shuan Ju and Thong, Kwai Lin and Ngoi, Soo Tein and Ahmad, Norazah and Nair, Gopinath Balakrish and Ramamurthy, Thandavarayan (2009) Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources. The Journal of General and Applied Microbiology, 55 (6). pp. 419-425. ISSN 0022-1260,

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Abstract

A pair of primers targeting the hlyA gene for Vibrio cholerae which could distinguish the classical from El Tor biotypes was designed and combined with other specific primers for ompW, rfb complex, and virulence genes such as ctxA, toxR, and tcpI in a multiplex PCR (m-PCR) assay. This m-PCR correctly identified 39 V. cholerae from clinical, water and seafood samples. The efficiency of this multiplex PCR (m-PCR) was compared with conventional biochemical and serogrouping methods. One O139 and 25 O1 V. cholerae strains including 10 environmental strains harbored all virulence-associated genes except 1 clinical strain which only had toxR and hlyA genes. Thirteen environmental strains were classified as non-O1/non-O139 and had the toxR and hlyA genes only. The detection limit of m-PCR was 7 × 104 cfu/ml. The m-PCR test was reliable and rapid and reduced the identification time to 4 h.

Item Type:	Article
Funders:	PS250/2008C, RG017-09BIO
Uncontrolled Keywords:	multiplex PCR; serogroup; Vibrio cholerae; virulence gene
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Ms Soo Tein Ngoi
Date Deposited:	22 Apr 2015 09:04
Last Modified:	21 Jul 2020 07:20
URI:	http://eprints.um.edu.my/id/eprint/13371

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