Chan, Kok Gan and Wong, Cheng Siang and Yin, Wai Fong and Sam, Choon Kook and Koh, Chong Lek (2010) Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacillus cereus isolate from Malaysian rainforest soil. Antonie Van Leeuwenhoek International Journal Of General And Molecular Microbiology, 98 (3). pp. 299-305.
Full text not available from this repository.Abstract
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria. KM1S was clustered closely to Bacillus cereus by 16S ribosomal DNA sequence analysis. It degraded N-3-oxo-hexanoyl homoserine lactone and N-3-oxo-octanoyl homoserine lactone in vitro rapidly at 4.98 and 6.56 mu g AHL h(-1) per 10(9) CFU/ml, respectively, as determined by the Rapid Resolution Liquid Chromatography. The aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of N-acylhomoserine lactones, of KM1S was amplified and cloned. Sequence analysis indicated the presence of the motif (106)HXDH-59 amino acids-H(169)-21 amino acids-D(191) for N-acylhomoserine lactone lactonases.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Uncontrolled Keywords: | Bacillus cereus; KG medium; Quorum sensing; Quorum quenching; N-acylhomoserine lactone; Rainforest soil |
| Subjects: | Q Science > Q Science (General) R Medicine |
| Depositing User: | Mr Faizal 2 |
| Date Deposited: | 20 Jan 2015 03:03 |
| Last Modified: | 23 Dec 2019 03:43 |
| URI: | http://eprints.um.edu.my/id/eprint/12272 |
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