Chan, K. (2009) Exochelin production in Mycobacterium neoaurum. International Journal of Molecular Sciences, 10 (1). pp. 345-53. ISSN 1661-6596, DOI 19333449.
Full text not available from this repository.Abstract
Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia. |
| Uncontrolled Keywords: | Mycobacterium neoaurum; Siderophore; Exochelin |
| Subjects: | R Medicine > RB Pathology |
| Depositing User: | Mr. Faizal Hamzah |
| Date Deposited: | 27 Apr 2011 03:30 |
| Last Modified: | 08 Jul 2017 05:42 |
| URI: | http://eprints.um.edu.my/id/eprint/1081 |
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