Ismail, I.S. and Muniandy, S. and Koon, C.Y. and Bee, J.C. and Rayappan, A.V. and Kum, T.W. and Qvist, R. (2008) Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR. Biomedical Research, 19 (2). pp. 117-124. ISSN 0388-6107,
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Quantification_of_Zonula_Occludens-1_mRNA_expression_in_cultu-red_bovine_retinal_endothelial_cells,_using_SYBR_green_real-time_PCR.pdf - Published Version Restricted to Repository staff only Download (546kB) | Request a copy |
Abstract
A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.
| Item Type: | Article |
|---|---|
| Funders: | UNSPECIFIED |
| Additional Information: | Department of Medicine, Faculty of Medicine, University of Malaya |
| Uncontrolled Keywords: | Bovine retinal endothelial cells; Zonula Occludens-1; Real time reverse transcription poly-merase chain reaction |
| Subjects: | R Medicine > R Medicine (General) |
| Divisions: | Faculty of Medicine |
| Depositing User: | Ms. Izzan Ramizah Idris |
| Date Deposited: | 29 Oct 2014 00:56 |
| Last Modified: | 29 Oct 2014 06:25 |
| URI: | http://eprints.um.edu.my/id/eprint/10273 |
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