Antidiabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells,ß-TC6 and its alleviating role in type 2 diabetic rats

Arya, Aditya and Looi, C.Y. and Cheah, S.C. and Mustafa, Mohd Rais (2012) Antidiabetic effects of Centratherum anthelminticum seeds methanolic fraction on pancreatic cells,ß-TC6 and its alleviating role in type 2 diabetic rats. Journal of Ethnopharmacology. pp. 1-36. ISSN 0378-8741,

Text 5.pdf - Published Version Restricted to Repository staff only Download (822kB) | Request a copy

Abstract

Ethnopharmacological relevance:Seeds of Centratherum anthelminticum (Asteraceae)have been popularly used in Ayurvedic medicine to treat diabetes and skin disorders.Folk medicine from Rayalaseema(Andhra Pradesh,India)reported wide spread usage in diabetes. Aim of the study:To investigate the hypoglycemic properties and mechanism of the me than olic fraction of Centratherum anthelminticum seeds(CAMFs)on mouse ß-TC6 pancreatic cell line and streptozotocin (STZ)induced diabetic rat models. Materials and Methods:We investigated the crude methanolic fraction of Centratherum an thelminticum seeds(CAMFs)on ß-TC6 cellline and confirmed its effects on type 1 and type 2 diabetic rats to understand its mechanism in managing diabetes mellitus.(CAMFs) was initially tested on ß-TC6 cells for cytotoxicity, 2 NBDG glucose uptake,insulin secretion and glucose transporter (GLUT1,2and4)protein expression.Furthermore,streptozotocin(STZ)–induced type 1 diabetic and STZ-nicotinamide–induced type2 diabetic rats were in traperitoneally (i.p)injected or administered orally with CAMFs daily for 28days.The effect of CAMFs on blood glucose and insulin levels was subsequently evaluated.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	corresponding auther Email:rais@um.edu.my
Uncontrolled Keywords:	Diabetes; Centratherum anthelminticum; Glucose uptake; Insulin secretion; GLUT2; GLUT4
Subjects:	R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine
Divisions:	Faculty of Medicine
Depositing User:	Ms. Norhamizah Tamizi
Date Deposited:	20 Oct 2014 03:14
Last Modified:	18 Dec 2019 06:42
URI:	http://eprints.um.edu.my/id/eprint/10063

Actions (login required)

View Item