Optimised conditions for PFGE separation of Pasteurella multocida B:2 DNA

Hasima, N.N. and Saidi, J. and Thong, Kwai Lin and Ansary, A. (2001) Optimised conditions for PFGE separation of Pasteurella multocida B:2 DNA. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 147-150.

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Abstract

Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.

Item Type: Article
Additional Information: Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Prof. Dr Kwai Lin Thong
Date Deposited: 12 Nov 2014 04:14
Last Modified: 12 Oct 2018 06:35
URI: http://eprints.um.edu.my/id/eprint/9969

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