Hasima, N.N. and Saidi, J. and Thong, Kwai Lin and Ansary, A. (2001) Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 147-150. ISSN 0128-7451,
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Abstract
Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.
Item Type: | Article |
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Funders: | UNSPECIFIED |
Additional Information: | Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
Uncontrolled Keywords: | Pasteurella multocida; PFGE; restriction enzymes |
Subjects: | Q Science > QR Microbiology |
Divisions: | Faculty of Science > Institute of Biological Sciences |
Depositing User: | Prof. Dr Kwai Lin Thong |
Date Deposited: | 12 Nov 2014 04:14 |
Last Modified: | 22 Oct 2021 01:41 |
URI: | http://eprints.um.edu.my/id/eprint/9969 |
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