Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA

Hasima, N.N. and Saidi, J. and Thong, Kwai Lin and Ansary, A. (2001) Optimised conditions for Pulsed Field Gel Electrophoresis (PFGE) separations of Pasteurella multocida B:2 DNA. Asia Pacific Journal of Molecular Biology and Biotechnology, 9 (2). pp. 147-150. ISSN 0128-7451,

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Abstract

Pulsed-field condition specific for Pasteurella multocida B:2 have been optimized to obtain a good separation resolution. Restriction enzyme, NotI, was the most suitable as it generates discernible number of bands. Other optimised electrophoretic conditions were: 1.5% agarose gel concentration, 200V, ramped pulse time of 1-25 s, and a runtime of 26 h. This research is important for fast and accurate characterisation of this bacterial strain following an outbreak in Malaysia.

Item Type:	Article
Funders:	UNSPECIFIED
Additional Information:	Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords:	Pasteurella multocida; PFGE; restriction enzymes
Subjects:	Q Science > QR Microbiology
Divisions:	Faculty of Science > Institute of Biological Sciences
Depositing User:	Prof. Dr Kwai Lin Thong
Date Deposited:	12 Nov 2014 04:14
Last Modified:	22 Oct 2021 01:41
URI:	http://eprints.um.edu.my/id/eprint/9969

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