Effect of carbon dioxide and bicarbonate as inorganic carbon sources on growth and adaptation of autohydrogenotrophic denitrifying bacteria

Ghafari, S.; Hasan, M.; Aroua, M.K. (2009) Effect of carbon dioxide and bicarbonate as inorganic carbon sources on growth and adaptation of autohydrogenotrophic denitrifying bacteria. Journal of Hazardous Materials, 162 (2-3). pp. 1507-1513. ISSN 0304-3894

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Abstract

Acclimation of autohydrogenotrophic denitrifying bacteria using inorganic carbon source (CO 2 and bicarbonate) and hydrogen gas as electron donor was performed in this study. In this regard, activated sludge was used as the seed source and sequencing batch reactor (SBR) technique was applied for accomplishing the acclimatization. Three distinct strategies in feeding of carbon sources were applied: (I) continuous sparging of CO 2, (II) bicarbonate plus continuous sparging of CO 2, and (III) only bicarbonate. The pH-reducing nature of CO 2 showed an unfavorable impact on denitrification rate; however bicarbonate resulted in a buffered environment in the mixed liquor and provided a suitable mean to maintain the pH in the desirable range of 7-8.2. As a result, bicarbonate as the only carbon source showed a faster adaptation, while carbon dioxide as the only carbon source as well as a complementary carbon source added to bicarbonate resulted in longer acclimation period. Adapted hydrogenotrophic denitrifying bacteria, using bicarbonate and hydrogen gas in the aforementioned pH range, caused denitrification at a rate of 13.33 mg NO 3 —N/g MLVSS/h for degrading 20 and 30 mg NO 3 —N/L and 9.09 mg NO 3 —N/g MLVSS/h for degrading 50 mg NO 3 —N/L.

Item Type: Article
Creators:
  1. Ghafari, S.
  2. Hasan, M.
  3. Aroua, M.K.(Department of Chemical Engineering, Faculty of Engineering Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
Journal or Publication Title: Journal of Hazardous Materials
Additional Information: Cited By (since 1996):14 Export Date: 21 April 2013 Source: Scopus CODEN: JHMAD :doi 10.1016/j.jhazmat.2008.06.039 PubMed ID: 18639979 Language of Original Document: English Correspondence Address: Aroua, M.K.; Department of Chemical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia; email: mkaroua@um.edu.my : Chemicals/CASbicarbonate, 144-55-8, 71-52-3; carbon dioxide, 124-38-9, 58561-67-4; nitrate, 14797-55-8; Bicarbonates; Carbon Dioxide, 124-38-9; Carbon, 7440-44-0; Sewage References: Terada, A., Hibiya, K., Nagai, J., Tsuneda, S., Hirata, A., Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofilm reactor applicable to high-strength nitrogenous wastewater treatment (2003) J. Biosci. 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Uncontrolled Keywords: Acclimation; Denitrification; Hydrogenotrophic; Nitrate; Water; Activated sludge process; Bacteria; Bacteriology; Batch reactors; Carbon dioxide; Hydrogen; Nitrification; Oxides; pH effects; Acclimation periods; Activated sludges; Autohydrogenotrophic denitrifying bacteria; Carbon sources; Denitrification rates; Denitrifying bacteria; Electron donors; Hydrogen gasses; Inorganic carbons; pH ranges; Seed sources; Sequencing batch reactors; bicarbonate; activated sludge; bacterium; growth response; inorganic carbon; adaptation; Physiological; article; bacterial growth; carbon source; denitrifying bacterium; nonhuman; pH measurement; Adaptation; Physiological; Bicarbonates; Carbon; Sewage.
Subjects: T Technology > TA Engineering (General). Civil engineering (General)
T Technology > TP Chemical technology
Divisions: Faculty of Engineering
Depositing User: Mr Jenal S
Date Deposited: 16 Jul 2013 14:41
Last Modified: 11 Dec 2013 10:46
URI: http://eprints.um.edu.my/id/eprint/7441

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