Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication

Wee, W.H. and Azhar Abdul Razak, M. and Kadri, N.A. (2012) Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication. Advanced Science Letters, 15 (1). pp. 1-4. ISSN 19366612 (ISSN)

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Abstract

A microfluidics-based device that has the capability to trap cells has been designed and constructed and its capabilities have been demonstrated using dielectrophoresis-a promising tool for the selective manipulation and separation of cells, bacteria and other bioparticles. The device consists of a dielectrophoretic chamber and an interdigitated electrode geometry that is fabricated from gold-coated glass slides. It was designed with the intention of providing a device that was compatible with standard laboratory equipment and had the capability to prevent leakage and discriminate cells. Experimental results reveal that the device is capable of trapping leukemic K562 cells at an average efficiency of 94.2. In addition to this, the design offers a number of advantages, which include the ability to exchange microscope slides and change separation chamber height. It is also reusable, easy to assembly and cleaning and provides a simple method of connecting electrodes and a power source. The device has the potential to become very beneficial in the preliminary stages of many diagnostic and immunosensing applications that require separation of different types of cells. © 2012 American Scientific Publishers All rights reserved.

Item Type: Article
Additional Information: Export Date: 11 December 2012 Source: Scopus Language of Original Document: English Correspondence Address: Kadri, N. A.; Department of Biomedical Engineering, Faculty of Engineering, University of Malaya, 50603 Kuala Lumpur, Malaysia References: Hughes, M., (2002) Electrophoresis, 23; Pethig, R., Markx, G., (1997) Trends Biotechnol., 15; Liu, B., Deng, Y., Qin, B.B., Li, Z.Y., (2011) Adv. Sci. Lett., 4, p. 1; Gascoyne, P., Huang, Y., Pethig, R., Vykoukal, J., Becker, F., (1992) Meas. Sci. Technol., 3; Masuda, S., Washizu, M., Iwadare, M., (1987) IEEE T. Ind. Appl., 23; Gascoyne, P., Wang, X., Huang, Y., Becker, F., (1997) IEEE T. Ind. Appl., 33; Gasperis, G., Yang, J., Becker, F., Gascoyne, P., Wang, X., (1999) Biomed. Microdevices, 2; Li, H., Bashir, R., (2002) Sensor. Actuat. B-Chem., 86; Doh, I., Cho, Y., (2005) Sensor. Actuat. A-Phys., 121; Labeed, F., Coley, H., Thomas, H., Hughes, M., (2003) Biophys. J., 85
Uncontrolled Keywords: BioMEMS Biotechnology Dielectrophoresis Microfluidics
Subjects: T Technology > T Technology (General)
Divisions: Faculty of Engineering
Depositing User: Mr. Mohammed Salim Abd Rahman
Date Deposited: 13 Mar 2013 07:09
Last Modified: 13 Mar 2013 07:09
URI: http://eprints.um.edu.my/id/eprint/5039

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