Yajit, Noor Liana Mat and Hashim, Noor Haza Fazlin and Illias, Rosli Mohd and Murad, Abdul Munir Abdul (2024) Expression and biochemical characterization of a novel thermostable alkaline β-1,3-1,4-glucanase (lichenase) from an alkaliphilic Bacillus lehensis G1. Protein Expression and Purification, 219. p. 106486. ISSN 1046-5928, DOI https://doi.org/10.1016/j.pep.2024.106486.
Full text not available from this repository.Abstract
New thermostable beta-1,3-1,4-glucanase (lichenase) designated as Blg29 was expressed and purified from a locally isolated alkaliphilic bacteria Bacillus lehensis G1. The genome sequence of B. lehensis predicted an open reading frame of Blg29 with a deduced of 249 amino acids and a molecular weight of 28.99 kDa. The gene encoding for Blg29 was successfully amplified via PCR and subsequently expressed as a recombinant protein using the E. coli expression system. Recombinant Blg29 was produced as a soluble form and further purified via immobilized metal ion affinity chromatography (IMAC). Based on biochemical characterization, recombinant Blg29 showed optimal activity at pH9 and temperature 60 degrees C respectively. This enzyme was stable for more than 2 h, incubated at 50 degrees C, and could withstand similar to 50 % of its activity at 70 degrees C for an hour and a half. No significant effect on Blg29 was observed when incubated with metal ions except for a small increase with ion Ca2+. Blg29 showed high substrate activity towards lichenan where V-m, K-m, K-cat,K- and k(cat)/K-m values were 2040.82 mu molmin(-1)mg(-1), 4.69 mg/mL, and 986.39 s(-1) and 210.32 mLs(-1)mg(-1) respectively. The high thermostability and activity make this enzyme useable for a broad prospect in industry applications.
| Item Type: | Article |
|---|---|
| Funders: | Ministry of Energy, Science, Technology, Environment and Climate Change (MESTECC), Malaysia (-10-05-16-MB002) |
| Uncontrolled Keywords: | Bacillus lehensis G1; Thermostable; beta-1,3-1,4-glucanase; Lichenase; Alkaliphilic |
| Subjects: | Q Science > Q Science (General) T Technology > T Technology (General) |
| Divisions: | Universiti Malaya |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 09 Jan 2025 04:04 |
| Last Modified: | 09 Jan 2025 04:04 |
| URI: | http://eprints.um.edu.my/id/eprint/46976 |
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