Antigenic cell associated Dengue 2 Virus proteins detected in Vitro using Dengue fever patients Sera

AbuBakar, S.; Azmi, A.; Misbah, S.; Chang, L.Y. (2002) Antigenic cell associated Dengue 2 Virus proteins detected in Vitro using Dengue fever patients Sera. Malaysian Journal of Pathology, 24 (1). pp. 29-36. ISSN 0126-8635

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Abstract

At least three major antigenic dengue 2 virus proteins were recognized by pooled dengue fever patients' sera in infected Aedes albopictus (C6/36) mosquito cells. Dengue virus envelope (E), premembrane (PrM) and non-structural protein 1 (NS 1) dimer were detected beginning on day 3 postinfection in both the cell membrane and cytosolic fractions. Using the patients' sera, the presence of antigenic intermediate core protein (C)-PrM and NS1-non-structural protein 2a (NS2a) in the cytoplasmic fraction of dengue 2 virus infected cells was revealed. The presence of a approximately 92 and approximately 84 kDa NS 1 dimer in the membrane (NS 1m) and cytosolic (NS 1c) fractions of C6/36 cells, respectively, was also recognized. Using individual patient's serum, it was further confirmed that all patients' sera contained antibodies that specifically recognized E, NS 1 and PrM present in the dengue 2 virus-infected cell membrane fractions, suggesting that these glycosylated virus proteins were the main antigenic proteins recognized in vivo. Detection of dengue 2 virus C antibody in some patients further suggested that C could be antigenic if presented in vivo.

Item Type: Article
Creators:
  1. AbuBakar, S.(Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
  2. Azmi, A.
  3. Misbah, S.
  4. Chang, L.Y.
Journal or Publication Title: Malaysian Journal of Pathology
Additional Information: Department of Medical Microbiology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
Uncontrolled Keywords: Dengue
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: miss munirah saadom
Date Deposited: 12 Nov 2012 09:33
Last Modified: 20 Oct 2014 15:19
URI: http://eprints.um.edu.my/id/eprint/3950

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