Osman, Norfaizah and Zahari, Azeana and Hazni, Hazrina and Othman, Wan Nurul Nazneem Wan and Rasol, Nurulfazlina Edayah and Ismail, Nor Hadiani and Champy, Pierre and Beniddir, Mehdi A. and Litaudon, Marc and Awang, Khalijah (2023) Isolation and characterization of compounds from ochreinauclea maingayi (Hook. f.) Ridsd. (Rubiaceae) with the aid of LCMS/MS molecular networking. Separations, 10 (2). ISSN 2297-8739,
Full text not available from this repository.Abstract
Phytochemical investigation of the dichloromethane crude extract from the bark of Ochreinauclea maingayi with the aid of LCMS/MS-based molecular networking guided the isolation and accelerated the elucidation of known and new indole alkaloids. The molecular networking analysis produces two main clusters, along with 41 non-prioritized clusters and self-loop nodes. Each cluster has several nodes which depict the fractions contained within those nodes. An implementation of a fraction mapping for each node represents the molecular weight and key fragment data of each compound. From the analysis of each cluster and node, we can deduce the indole alkaloids are the scaffold of interest. Indole scaffold can be found between F5 and F10 that contain several types of indole alkaloids. In total, we have successfully purified nine indole alkaloids, including 9H-beta-carboline-4-carboxylate 2, norharmane 3, harmane 4, naucledine 10, neonaucline 15, 1,2,3,4-tetranorharmane-1-one 16, naulafine 19, cadambine 9, and a new monoterpene indole alkaloid dihyrodeglycocadambine 7 from F5 to F10 using a chromatographic technique. Their structures were confirmed by 1D-NMR, 2D-NMR, UV, IR, LCMS, and MS2LDA. Several clusters and nodes contain ions that could not be annotated, suggesting that they may possess novel compounds that are yet to be discovered.
| Item Type: | Article |
|---|---|
| Funders: | None |
| Uncontrolled Keywords: | Indole alkaloid; Molecular networking; Dereplication; Rubiaceae; Ochreinaculea maingayi |
| Subjects: | Q Science > Q Science (General) T Technology > T Technology (General) |
| Divisions: | Universiti Malaya |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 30 Sep 2025 07:37 |
| Last Modified: | 30 Sep 2025 07:37 |
| URI: | http://eprints.um.edu.my/id/eprint/38727 |
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