SpeI restriction enzyme displays greater discriminatory power than XbaI enzyme does in a pulsed-field gel electrophoresis study on 146 clinical burkholderia pseudomallei isolates

Chua, K.H.; See, K.H.; Thong, K.L.; Puthucheary, S.D. (2011) SpeI restriction enzyme displays greater discriminatory power than XbaI enzyme does in a pulsed-field gel electrophoresis study on 146 clinical burkholderia pseudomallei isolates. Jpn. J. Infect. Dis, 64 (3). pp. 228-233.

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    Abstract

    Restriction enzymes SpeI and XbaI were used in a pulsed-field gel electrophoresis (PFGE) study for molecular characterization of 146 clinical Burkholderia pseudomallei isolates. The PFGE parameters were optimized to enable comparable, reproducible, and robust results. The optimized parameters for both SpeI and XbaI restriction enzymes used in this study were 200 V and a pulse time of 5 to 65 s for a 28-h runtime. Using SpeI, 9 different clusters were identified, whereas 6 clusters were identified by XbaI digestion, which exhibited 85 similarity to SpeI. SpeI (discrimination index D=0.854) showed higher discriminatory power than XbaI did (D=0.464).

    Item Type: Article
    Creators:
    1. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. See, K.H.
    3. Thong, K.L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    4. Puthucheary, S.D.
    Journal or Publication Title: Jpn. J. Infect. Dis
    Additional Information: Corresponding author: Mailing address: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. Tel: +603- 79676607, Fax: +603-79676600, E-mail: khchua@um.edu.my
    Uncontrolled Keywords: Molecular Medicine; Restriction enzymes SpeI and XbaI
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 28 Sep 2012 09:55
    Last Modified: 15 Dec 2014 14:51
    URI: http://eprints.um.edu.my/id/eprint/3850

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