Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach. l

Chua, K.H.; Kee, B.; Thong, K. L. (2009) Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach. l. African Journal of Biotechnology, 8 (18). pp. 4623-4626. ISSN 1684-5315

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    Abstract

    A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual.

    Item Type: Article
    Journal or Publication Title: African Journal of Biotechnology
    Additional Information: Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA
    Uncontrolled Keywords: Salmonella enterica serovar Typhi, recombinant protein, Vi mimotope.
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 01 Nov 2012 15:57
    Last Modified: 21 Jan 2014 16:31
    URI: http://eprints.um.edu.my/id/eprint/3789

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