Chua, K.H.; Kee, B.; Thong, K. (2009) Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach. l. African Journal of Biotechnology, 8 (18). pp. 4623-4626. ISSN 1684-5315
Abstract
A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual.
| Item Type: | Article |
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| Creators: | - Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
- Kee, B.
- Thong, K.
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| Journal or Publication Title: | African Journal of Biotechnology |
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| Additional Information: | Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA |
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| Uncontrolled Keywords: | Salmonella enterica serovar Typhi, recombinant protein, Vi mimotope. |
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| Subjects: | R Medicine |
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| Divisions: | Faculty of Medicine |
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| Depositing User: | Ms azrahani halim |
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| Date Deposited: | 01 Nov 2012 15:57 |
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| Last Modified: | 01 Nov 2012 15:57 |
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| URI: | http://eprints.um.edu.my/id/eprint/3789 |
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