Chua, K.H.; Kee, B.; Thong, K. L. (2009) Cloning and expression of a Vi mimotope of Salmonella enterica serovar Typhi through nucleotide-nucleotide hybridization approach. l. African Journal of Biotechnology, 8 (18). pp. 4623-4626. ISSN 1684-5315
A recombinant His-Vi protein of Salmonella enterica serovar Typhi was successfully constructed and cloned into an expression vector through a nucleotide-nucleotide hybridization approach. After transformation of the construct into Escherichia coli, the recombinant His-Vi protein with a size of approximately 4 kDa was successfully produced and proven by Western blot analysis. This recombinant protein can be used to detect specific anti-Vi antibody produced by typhoid patients. Overall, the His-Vi recombinant protein could serve as a potential diagnostic reagent to detect S. Typhi infection in an individual.
|Item Type: ||Article|
- Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
- Kee, B.
- Thong, K. L.(Institute of Biological Sciences, Faculty of Science Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
|Journal or Publication Title: ||African Journal of Biotechnology|
|Additional Information: ||Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA|
|Uncontrolled Keywords: ||Salmonella enterica serovar Typhi, recombinant protein, Vi mimotope.|
|Subjects: ||R Medicine|
|Divisions: ||Faculty of Medicine|
|Depositing User: ||Ms azrahani halim|
|Date Deposited: ||01 Nov 2012 15:57|
|Last Modified: ||21 Jan 2014 16:31|
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