Application of genefishing discovery system on differential gene expression study for prokaryotic system

Chua, K.H.; Chai, H.C.; Puthucheary, S.D. (2010) Application of genefishing discovery system on differential gene expression study for prokaryotic system. African Journal of Biotechnology, 9 (35). pp. 5629-5631. ISSN 1684-5315

[img]
Preview
PDF - Published Version [error in script]
Download (70Kb) | Preview

    Abstract

    GeneFishing Discovery is a system applied on disease and gene related research for eukaryotes and the starting material for the study could be either total RNA or mRNA. In this study we demonstrate the use of this discovery system for a prokaryotic system by modifying the eukaryotic protocol using the poly (A)-tailing approach. As an example we compared the gene expression pattern of two closely related species of bacteria under the same family (Burkholderia thailandensis and Burkholderia cepacia) with satisfactory results.

    Item Type: Article
    Creators:
    1. Chua, K.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Chai, H.C.
    3. Puthucheary, S.D.
    Journal or Publication Title: African Journal of Biotechnology
    Additional Information: Corresponding author. E-mail: khchua@um.edu.my. Tel: 603- 79676607. Fax: 603-79676600.
    Uncontrolled Keywords: Genefishing, differential expression, prokaryotic system.
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms azrahani halim
    Date Deposited: 25 Oct 2012 10:11
    Last Modified: 25 Oct 2012 10:11
    URI: http://eprints.um.edu.my/id/eprint/3780

    Actions (For repository staff only: Login required)

    View Item

    Document Downloads

    More statistics for this item...