Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies

Mohamed, E.; Abdul-Rahman, P.S.; Doustjaialil, S.R.; Chen, Y.; Lim, B.K.; Omar, S.Z.; Bustam, A.Z.; Singh, V.A.; Mohd-Taib, N.; Yip, C.H.; Hashim, O.H. (2008) Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies. Electrophoresis, 29 (12). pp. 2645-2650. ISSN 0173-0835

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    Abstract

    A 35 kDa glycoprotein whose abundance was previously demonstrated to be enhanced in sera of patients with endometrial adenocarcinoma (n = 12), was isolated from pooled sera of three of the cancer patients using champedak galactose-binding lectin affinity chromatography in the present study. Subjecting it to 2-DE and MS/MS, the glycoprotein was identified as the O-glycosylated fragment of inter-a-trypsin inhibitor heavy chain H4 (ITIH4). When compared to control sera (n = 17), expression of the 35 kDa ITIH4 cleavage fragment was demonstrated to be significantly enhanced in sera of patients with breast carcinoma (n = 10), epithelial ovarian carcinoma (n = 10), and germ cell ovarian carcinoma (n = 10) but not in patients with nasopharyngeal carcinoma (n = 13) and osteosarcoma (n = 7). The lectin-based electrophoretic bioanalytical method adopted in the present study may be used to assess the physiological relevance of ITIH4 fragmentation and its correlation with different malignancies, their stages and progression.

    Item Type: Article
    Creators:
    1. Mohamed, E.
    2. Abdul-Rahman, P.S.
    3. Doustjaialil, S.R.
    4. Chen, Y.
    5. Lim, B.K.
    6. Omar, S.Z.
    7. Bustam, A.Z.
    8. Singh, V.A.
    9. Mohd-Taib, N.
    10. Yip, C.H.
    11. Hashim, O.H.(Department of Molecular Medicine, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    Journal or Publication Title: Electrophoresis
    Additional Information: Correspondence: Professor Onn Haji Hashim, Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: onnhashim@um.edu.my Fax: 1603-79474957
    Uncontrolled Keywords: Cancer; Champedak; Inter-a-trypsin inhibitor heavy chain H4; Lectin; Serum
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Ms Haslinda Lahuddin
    Date Deposited: 10 Jul 2012 09:23
    Last Modified: 11 Dec 2013 11:45
    URI: http://eprints.um.edu.my/id/eprint/3436

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