Jhuma, Tania Akter and Rafeya, Jannatul and Sultana, Shahnaz and Rahman, Mohammad Tariqur and Karim, Muhammad Manjurul (2021) Isolation of endophytic salt-tolerant plant growth-promoting rhizobacteria from Oryza sativa and evaluation of their plant growth-promoting traits under salinity stress condition. Frontiers in Sustainable Food Systems, 5. ISSN 2571-581X, DOI https://doi.org/10.3389/fsufs.2021.687531.
Full text not available from this repository.Abstract
The application of plant growth-promoting rhizobacteria (PGPR) as vital components for plant growth promotion against biotic and abiotic stresses could be a promising strategy to improve crop production in areas vulnerable to increasing salinity. Here, we isolated Seventy-five endophytic bacteria from roots of healthy Oryza sativa grown in a saline environment of the southern coastal region of Bangladesh. The endophytes in a culture of similar to 10(8) CFU/ml showed arrays of plant growth-promoting (PGP) activities: phytohormone (Indole acetic acid) production (1.20-60.13 mu g/ml), nutrient (phosphate) solubilization (0.02-1.81 mu g/ml) and nitrogen fixation (70.24-198.70 mu g/ml). Four genomically diverse groups were identified namely, Enterobacter, Achromobacter, Bacillus, and Stenotrophomonas using amplified ribosomal DNA restriction analysis followed by their respective 16S rDNA sequence analyses with that of the data available in NCBI GenBank. These four specific isolates showed tolerance to NaCl ranging from 1.37 to 2.57 mol/L in the nutrient agar medium. Under a 200 mmol/L salt stress in vitro, the bacteria in a culture of 10(8) CFU/ml exhibited competitive exopolysaccharide (EPS) production: Stenotrophomonas (65 mu g/ml) and Bacillus (28 mu g/ml), when compared to the positive control, Pseudomonas spp. (23.65 mu g/ml), a phenomenon ably supported by their strong biofilm-producing abilities both in a microtiter plate assay, and in soil condition; and demonstrated by images of the scanning electron microscope (SEM). Overall, the isolated endophytic microorganisms revealed potential PGP activities that could be supported by their biofilm-forming ability under salinity stress, thereby building up a sustainable solution for ensuring food security in coastal agriculture under changing climate conditions.
| Item Type: | Article |
|---|---|
| Funders: | University Grants Commission, India (6(75)/UGC/S&T/Bio-05/2018/2732) |
| Uncontrolled Keywords: | Biofilm; endophytes; Plant growth-promoting rhizobacteria; Salt-tolerant; Sustainable agricultural production |
| Subjects: | Q Science > Q Science (General) R Medicine > RK Dentistry T Technology > T Technology (General) |
| Divisions: | Faculty of Dentistry |
| Depositing User: | Ms Zaharah Ramly |
| Date Deposited: | 22 Jun 2022 07:58 |
| Last Modified: | 22 Jun 2022 07:58 |
| URI: | http://eprints.um.edu.my/id/eprint/27976 |
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