Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris

Fong, M. Y.; Lau, Y. L. (2004) Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris. Parasitology Research, 92 (2). pp. 173-176.

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    Abstract

    A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with differentparasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.

    Item Type: Article
    Creators:
    1. Fong, M. Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
    2. Lau, Y. L.
    Journal or Publication Title: Parasitology Research
    Additional Information: M.-Y. Fong (&) Æ Y.-L. Lau Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: fongmy@um.edu.my Fax: +60-3-79674754
    Uncontrolled Keywords: Human Toxocariasis
    Subjects: R Medicine
    Divisions: Faculty of Medicine
    Depositing User: Prof. Dr. Mun Yik Fong
    Date Deposited: 20 Feb 2012 09:10
    Last Modified: 20 Feb 2012 09:10
    URI: http://eprints.um.edu.my/id/eprint/2702

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