Fong, M.Y.; Lau, Y.L. (2004) Recombinant expression of the larval excretory-secretory antigen TES-120 of Toxocara canis in the methylotrophic yeast Pichia pastoris. Parasitology Research, 92 (2). pp. 173-176.
A gene encoding the larval excretory-secretory antigen TES-120 of the dog ascarid worm Toxocara canis was cloned into the methylotrophic yeast Pichia pastoris. Specificity of the recombinant TES-120 antigen produced by the yeast was investigated. Forty-five human serum samples from patients infected with differentparasitic organisms, including 8 cases of toxocariasis, were tested against the recombinant antigen in immunoblot assays. Results from the assays showed that the recombinant TES-120 antigen reacted with sera from toxocariasis patients only. This highly specific recombinant TES-120 antigen can potentially be used for the development of an inexpensive serodiagnostic assay for human toxocariasis.
|Item Type: ||Article|
- Fong, M.Y.(Department of Parasitology, Faculty of Medicine Building, University of Malaya, 50603 Kuala Lumpur, MALAYSIA)
- Lau, Y.L.
|Journal or Publication Title: ||Parasitology Research|
|Additional Information: ||M.-Y. Fong (&) Æ Y.-L. Lau Department of Parasitology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia E-mail: firstname.lastname@example.org Fax: +60-3-79674754|
|Uncontrolled Keywords: ||Human Toxocariasis|
|Subjects: ||R Medicine|
|Divisions: ||Faculty of Medicine|
|Depositing User: ||Prof. Dr. Mun Yik Fong|
|Date Deposited: ||20 Feb 2012 09:10|
|Last Modified: ||20 Oct 2014 10:57|
Actions (For repository staff only: Login required)