Thung, Tze Young and Lee, Epeng and Mahyudin, Nor Ainy and Wan Mohamed Radzi, Che Wan Jasimah and Mazlan, Nurzafirah and Tan, Chia Wanq and Radu, Son (2020) Partial characterization and in vitro evaluation of a lytic bacteriophage for biocontrol of Campylobacter jejuni in mutton and chicken meat. Journal of Food Safety, 40 (2). e12770. ISSN 0149-6085, DOI https://doi.org/10.1111/jfs.12770.
Full text not available from this repository.Abstract
Campylobacter jejuni is a major foodborne pathogen causing acute bacterial enteritis worldwide. Screening of novel bacteriophages as biocontrol tools to eliminate C. jejuni in food during pre- and postharvest is an emerging research field. In this study, we partially characterized C. jejuni bacteriophage CJ01 for potential use as biocontrol agent. Transmission electron microscopy revealed bacteriophage CJ01 belonged to the Myoviridae family with a genome size of approximately 136 kb. The results of host range assay revealed CJ01 was able to infect C. jejuni and C. lari strains, with burst size about 35 phages per infected cell and latent period of 30 min. The effectiveness of bacteriophage CJ01 (with 100 multiplicity of infection = 100) was determined in mutton and chicken meat artificially contaminated with C. jejuni. The amount of C. jejuni in treated mutton and chicken meat were significantly reduced by 1.70 log CFU/g and 1.68 log CFU/g, respectively, after 48 hr stored at refrigeration temperature (4°C). The data presented here suggest that bacteriophage CJ01 can be used as a postharvest biocontrol agent. © 2020 Wiley Periodicals, Inc.
| Item Type: | Article |
|---|---|
| Funders: | Fundamental Research Grant Scheme (FRGS) of the Ministry of Higher Education (MOHE), Malaysia (01-01-18-2015FR) |
| Uncontrolled Keywords: | Bacteriophages; Bacteria; Phage treatment |
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology T Technology > T Technology (General) |
| Divisions: | Faculty of Science > Department of Science and Technology Studies |
| Depositing User: | Ms. Juhaida Abd Rahim |
| Date Deposited: | 15 Jun 2020 01:02 |
| Last Modified: | 15 Jun 2020 01:02 |
| URI: | http://eprints.um.edu.my/id/eprint/24808 |
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