Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis

Bao, Xiucui and Huo, Gaoxiang and Li, Li and Cao, Xuebin and Liu, Yamei and Lakshmipriya, Thangavel and Chen, Yeng and Hariri, Firdaus and Gopinath, Subash Chandra Bose (2019) Coordinated Dispersion and Aggregation of Gold Nanorod in Aptamer-Mediated Gestational Hypertension Analysis. Journal of Analytical Methods in Chemistry, 2019. p. 5676159. ISSN 2090-8865

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Abstract

Gestational hypertension is one of the complicated disorders during pregnancy; it causes the significant risks, such as placental abruption, neonatal deaths, and maternal deaths. Hypertension is also responsible for the metabolic and cardiovascular issues to the mother after the years of pregnancy. Identifying and treating gestational hypertension during pregnancy by a suitable biomarker is mandatory for the healthy mother and foetus development. Cortisol has been found as a steroid hormone that is secreted by the adrenal gland and plays a pivotal role in gestational hypertension. A normal circulating level of cortisol is involved in the regulation of blood pressure, and it is necessary to monitor the changes in the level of cortisol during pregnancy. In this work, aptamer-based colorimetric assay is demonstrated as a model with gold nanorod to quantify the level of cortisol using the coordinated aggregation (at 500 mM of NaCl) and dispersion (with 10 mu M of aptamer), evidenced by the scanning electron microscopy observation and UV-visible spectroscopy analysis. This colorimetric assay is an easier visual detection and reached the limit of detection of cortisol at 0.25 mg/mL. This method is reliable to identify the condition of gestational hypertension during the pregnancy period.

Item Type: Article
Uncontrolled Keywords: colorimetric detection; cortisol; nanoparticle; biosensor; pregnancy; assay; preeclampsia; sensitivity; blocking; Chemistry; Engineering
Subjects: R Medicine
R Medicine > RC Internal medicine
Divisions: Faculty of Dentistry > Dept of Oral Biology
Depositing User: Mr Ahmad Azwan Azman
Date Deposited: 21 Feb 2020 02:01
Last Modified: 21 Feb 2020 02:01
URI: http://eprints.um.edu.my/id/eprint/23880

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