Multiplex polymerase chain reaction-restriction fragment length polymorphism assay discriminates of rabbit, rat and squirrel meat in frankfurter products

Ali, Md. Eaqub and Ahamad, Mohammad Nasir Uddin and Asing, - and Hossain, M.A. Motalib and Sultana, Sharmin (2018) Multiplex polymerase chain reaction-restriction fragment length polymorphism assay discriminates of rabbit, rat and squirrel meat in frankfurter products. Food Control, 84. pp. 148-158. ISSN 0956-7135

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Official URL: https://doi.org/10.1016/j.foodcont.2017.07.030

Abstract

The demands for rabbit meat are rapidly growing and Rabbitry is becoming a mean of livelihood for many youths. Rats and squirrels are very close relatives of rabbits, could be hunted freely or raised in domestic farming and so could be substituted in expensive rabbit meat. This study, for the first time, developed and validated a tetraplex polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay to identify and discriminate rabbit, rat and squirrel meat under raw and processed foods. Four sets of primes amplified 123, 108, 243, and 141 bp fragments from rabbit, rat, squirrel and all eukaryotes, respectively. Specificity was confirmed through sequencing and RFLP analysis. When PCR products were digested with BtsIMutI and BtsCI enzymes, distinctive fingerprints (115 & 8 bp for rabbit; 64 & 44 bp for rat and 176 & 67 bp for squirrel) were obtained. The detection limit of the assay was 0.1% meat in frankfurter formulation.

Item Type: Article
Uncontrolled Keywords: Rabbitry; Tetraplex PCR-RFLP; Processed foods; Fingerprints; Restriction enzymes
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QH Natural history
T Technology > T Technology (General)
Divisions: Deputy Vice Chancellor (Research & Innovation) Office > Centre for Research in Biotechnology for Agriculture
Deputy Vice Chancellor (Research & Innovation) Office > Nanotechnology & Catalysis Research Centre
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 25 Sep 2019 07:26
Last Modified: 25 Sep 2019 07:26
URI: http://eprints.um.edu.my/id/eprint/22558

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