Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer

Lin, Yu Kiat and Show, Pau Loke and Yap, Yee Jiun and Ariff, Arbakariya and Annuar, Mohamad Suffian Mohamad and Lai, Oi Ming and Ling, Tau Chuan and Ng, Eng Poh (2018) Extractive Bioconversion of Gamma-Cyclodextrin and Recycling of Cyclodextrin Glycosyltransferase in Liquid Biphasic System Using Thermo-Separating Polymer. Frontiers in Chemistry, 6. p. 448. ISSN 2296-2646

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Official URL: https://doi.org/10.3389/fchem.2018.00448

Abstract

An extractive bioconversion conducted on soluble starch with cyclodextrin glycosyltransferase (CGTase) enzyme in ethylene oxide-propylene oxide (EOPO)/potassium phosphates liquid biphasic system (LBS) to extract gamma-cyclodextrin (γ-CD) was examined. A range of EOPO (with potassium phosphates) molecular weights was screen to investigate the effect of the latter on the partioning efficency of CGTase and γ-CD. The results show that the optimal top phase γ-CD yield (74.4%) was reached in 35.0% (w/w) EOPO 970 and 10.0% (w/w) potassium phosphate with 2.0% (w/w) sodium chloride. A theoretical explanation for the effect of NaCl on γ-CD was also presented. After a 2 h bioconversion process, a total of 0.87 mg/mL concentration of γ-CD was produced in the EOPO/ phosphates LBS top phase. After the extraction of top phase from LBS, four continuous repetitive batches were successfully conducted with relative CGTase activity of 1.00, 0.86, 0.45, and 0.40 respectively.

Item Type: Article
Uncontrolled Keywords: Bacillus cereus; Cyclodextrin; Ethylene oxide-propylene oxide; Extractive bioconversion; Liquid biphasic system
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
T Technology > TA Engineering (General). Civil engineering (General)
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 30 May 2019 05:05
Last Modified: 30 May 2019 05:05
URI: http://eprints.um.edu.my/id/eprint/21423

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