Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana

Gan, Khai Swan and Tan, Chon Seng and Othman, Rofina Yasmin and Harikrishna, Jennifer Ann (2018) Transient Expression of an Immunogenic Envelope Attachment Glycoprotein of Nipah Virus in Nicotiana benthamiana. Sains Malaysiana, 47 (3). pp. 499-509. ISSN 0126-6039

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Official URL: https://doi.org/10.17576/jsm-2018-4703-09

Abstract

The Nipah virus is highly virulent to swine and humans. The envelope attachment glycoprotein (G) of Nipah virus plays a key role in viral entry and induction of neutralizing antibody in mammalian hosts, thus is considered a good candidate for vaccine development. Plant transient expression systems are gaining recognition as a viable alternative for the production of vaccine antigens. In this study, we expressed the Nipah virus G protein heterologously in Nicotiana benthamiana using an agroinfiltration approach. The highest expression of recombinant G protein in N. benthamiana at RNA and protein levels was detected on day 9 post-infiltration. Western blot analysis demonstrated that the purified G protein reacted specifically with rabbit anti-Nipah Virus serum, indicating its potential for vaccine use.

Item Type: Article
Uncontrolled Keywords: Biotechnology; KDEL; plants; tobacco PR1a; vaccine
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 04 Mar 2019 08:16
Last Modified: 04 Mar 2019 08:17
URI: http://eprints.um.edu.my/id/eprint/20589

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