Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA

Tan, Suh Nih and Lim, Hong Chang and Teng, Sing Tung and Lim, Po Teen and Leaw, Chui Pin (2015) Pseudo-nitzschia species (Bacillariophyceae) identification and delineation using mitochondrial cox1 gene sequences as compared to LSU rDNA. Fisheries Science, 81 (5). pp. 831-838. ISSN 0919-9268

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Official URL: http://dx.doi.org/10.1007/s12562-015-0902-7

Abstract

An attempt was made to infer the phylogeny of Pseudo-nitzschia species by using the mitochondrial-encoded gene, cytochrome c oxidase subunit I (cox1), and comparing it with the nuclear-encoded large subunit ribosomal DNA (LSU rDNA). A pair of primers targeting Pseudo-nitzschia cox1 was designed in silico and used to infer the molecular phylogeny of Pseudo-nitzschia. The primer pair was tested using genomic DNAs isolated from six species of Pseudo-nitzschia from Malaysia. The phylogenetic inference of cox1 was then compared to the LSU rDNA phylogeny. Phylogenetic reconstructions of both data sets revealed monophyly of Pseudo-nitzschia species complexes. The range of genetic divergences among Pseudo-nitzschia species were higher in the cox1 data set (3.5–20.4 %) compared to the LSU rDNA data set (0.1–8.8 %). The present study suggests that high genetic divergence in cox1 of Pseudo-nitzschia species could be a useful genetic marker for DNA bar coding.

Item Type: Article
Uncontrolled Keywords: cox1; Diatom; LSU rDNA; Primer; Pseudo-nitzschia
Subjects: Q Science > Q Science (General)
Q Science > QH Natural history
Divisions: Faculty of Science
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 05 Oct 2018 05:28
Last Modified: 05 Oct 2018 05:28
URI: http://eprints.um.edu.my/id/eprint/19614

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