Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.

Thirugnanasampandan, R. and Ramya, G. and Gogulramnath, M. and Rajarajeswaran, J. and Kanthimathi, M.S. (2014) Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil. Pharmacognosy Journal, 7 (1). pp. 32-36. ISSN 0975-3575

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Official URL: http://dx.doi.org/10.5530/pj.2015.7.3

Abstract

Introduction: Plectranthus amboinicus(Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53±0.01 and 87±0.01μg/mL respectively. At 200μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.

Item Type: Article
Uncontrolled Keywords: Essential oil; Cytotoxicity; Antigenotoxicity; Zymogram; RT- PCR
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 03 Oct 2018 03:25
Last Modified: 03 Oct 2018 03:25
URI: http://eprints.um.edu.my/id/eprint/19550

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