A cell-based screening system for anti-influenza A virus agents

Wong, W.Y. and Loh, S.W. and Ng, W.L. and Tan, M.C. and Yeo, K.S. and Looi, C.Y. and Maah, M.J. and Ea, C.K. (2015) A cell-based screening system for anti-influenza A virus agents. Scientific Reports, 5 (1). p. 8672. ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/srep08672

Abstract

Emerging of drug resistant influenza A virus (IAV) has been a big challenge for anti-IAV therapy. In this study, we describe a relatively easy and safe cell-based screening system for anti-IAV replication inhibitors using a non-replicative strain of IAV. A nickel (II) complex of polyhydroxybenzaldehyde N4-thiosemicarbazone (NiPT5) was recently found to exhibit anti-inflammatory activity in vivo and in vitro. NiPT5 impedes the signaling cascades that lead to the activation of NF-κB in response to different stimuli, such as LPS and TNFα. Using our cell-based screening system, we report that pretreating cells with NiPT5 protects cells from influenza A virus (IAV) and vesicular stomatitis virus (VSV) infection. Furthermore, NiPT5 inhibits replication of IAV by inhibiting transcription and translation of vRNAs of IAV. Additionally, NiPT5 reduces IAV-induced type I interferon response and cytokines production. Moreover, NiPT5 prevents activation of NF-κB, and IRF3 in response to IAV infection. These results demonstrate that NiPT5 is a potent antiviral agent that inhibits the early phase of IAV replication.

Item Type: Article
Uncontrolled Keywords: Antiviral Agents; Cell Line, Tumor; Cytokines; Drug Evaluation, Preclinical; Humans; Influenza A virus; Interferon Regulatory Factor-3; Interferons; NF-kappa B; Virus Replication
Subjects: Q Science > QD Chemistry
Q Science > QH Natural history
R Medicine
Divisions: Faculty of Medicine
Faculty of Science > Dept of Chemistry
Faculty of Science > Institute of Biological Sciences
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 26 Sep 2018 07:57
Last Modified: 26 Sep 2018 07:57
URI: http://eprints.um.edu.my/id/eprint/19434

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