Dysbiosis of the microbiome in gastric carcinogenesis

Castaño-Rodríguez, N. and Goh, K.L. and Fock, K.M. and Mitchell, H.M. and Kaakoush, N.O. (2017) Dysbiosis of the microbiome in gastric carcinogenesis. Scientific Reports, 7 (1). p. 15957. ISSN 2045-2322

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Official URL: http://dx.doi.org/10.1038/s41598-017-16289-2

Abstract

The gastric microbiome has been proposed as an etiological factor in gastric carcinogenesis. We compared the gastric microbiota in subjects presenting with gastric cancer (GC, n = 12) and controls (functional dyspepsia (FD), n = 20) from a high GC risk population in Singapore and Malaysia. cDNA from 16S rRNA transcripts were amplified (515F-806R) and sequenced using Illumina MiSeq 2 × 250 bp chemistry. Increased richness and phylogenetic diversity but not Shannon's diversity was found in GC as compared to controls. nMDS clustered GC and FD subjects separately, with PERMANOVA confirming a significant difference between the groups. H. pylori serological status had a significant impact on gastric microbiome α-diversity and composition. Several bacterial taxa were enriched in GC, including Lactococcus, Veilonella, and Fusobacteriaceae (Fusobacterium and Leptotrichia). Prediction of bacterial metabolic contribution indicated that serological status had a significant impact on metabolic function, while carbohydrate digestion and pathways were enriched in GC. Our findings highlight three mechanisms of interest in GC, including enrichment of pro-inflammatory oral bacterial species, increased abundance of lactic acid producing bacteria, and enrichment of short chain fatty acid production pathways.

Item Type: Article
Uncontrolled Keywords: Dysbiosis; Microbiome; Gastric carcinogenesis
Subjects: R Medicine
Divisions: Faculty of Medicine
Depositing User: Ms. Juhaida Abd Rahim
Date Deposited: 13 Sep 2018 03:47
Last Modified: 13 Sep 2018 03:47
URI: http://eprints.um.edu.my/id/eprint/19217

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