Exochelin production in Mycobacterium neoaurum.

Chan, K. (2009) Exochelin production in Mycobacterium neoaurum. International journal of molecular sciences, 10 (1). pp. 345-53. ISSN 1422-0067

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Abstract

Mycobacterium neoaurum is a soil saprophyte and obligate aerobic bacterium. This group of mycobacterium is relatively fast-growing. They form colonies on nutrient agar at 37 masculineC within 3 - 4 days. In natural soil habitats, bioavailability of iron is limited. To facilitate iron uptake, most mycobacteria produce siderophores. One example is exochelin, which is extracellular and water-soluble. In this report, the production of exochelin in M. neoaurum was induced in iron-deficiency, but repressed under ironsufficiency growth conditions. It is however not induced under zinc-deficiency growth conditions. The growth of this mycobacterium was correlated with exochelin secretion under iron-deficiency culture conditions. When M. neoaurum was grown in defined medium containing 0.04 microg Fe(III)/mL (final concentration), the production of exochelin reached a maximum and the corresponding cell growth was comparable to that under iron-sufficiency conditions. In this study, exochelin was purified from spent supernatant of M. neoaurum by semi-preparative chromatography. When saturated ferric chloride solution was added into the purified exochelin, a ferri-exochelin complex was formed. It is proposed that iron uptake in M. neoaurum is exochelin-mediated.

Item Type: Article
Creators:
  1. Chan, K.
Journal or Publication Title: International journal of molecular sciences
Additional Information: Institute of Biological Sciences, University of Malaya, Kuala Lumpur, Malaysia.
Uncontrolled Keywords: Mycobacterium neoaurum; Siderophore; Exochelin
Subjects: R Medicine > RB Pathology
Divisions: UNSPECIFIED
Depositing User: Mr. Faizal Hamzah
Date Deposited: 27 Apr 2011 11:30
Last Modified: 27 Apr 2011 11:30
URI: http://eprints.um.edu.my/id/eprint/1081

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