Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR

Ismail, I.S. and Muniandy, S. and Koon, C.Y. and Bee, J.C. and Rayappan, A.V. and Kum, T.W. and Qvist, R. (2008) Quantification of Zonula Occludens-1 mRNA expression in cultu-red bovine retinal endothelial cells, using SYBR green real-time PCR. Biomedical Research, 19 (2). pp. 117-124. ISSN 0388-6107

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Abstract

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10-7M to 10-9 M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohisto-chemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.

Item Type: Article
Additional Information: Department of Medicine, Faculty of Medicine, University of Malaya
Uncontrolled Keywords: Bovine retinal endothelial cells; Zonula Occludens-1; Real time reverse transcription poly-merase chain reaction
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine
Depositing User: Ms. Izzan Ramizah Idris
Date Deposited: 29 Oct 2014 00:56
Last Modified: 29 Oct 2014 06:25
URI: http://eprints.um.edu.my/id/eprint/10273

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